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BCR::ABL1 IS
CALIBRATION

MULTI-POINT INTERNATIONAL SCALE CALCULATOR

Professional-grade BCR::ABL1 IS calibration tools for consistent patient monitoring across laboratories worldwide.

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TOOL SPOTLIGHT

BCR-ABL1 IS MULTI-POINT CALIBRATION CALCULATOR REVOLUTIONIZES LABORATORY STANDARDIZATION

Our International Scale calibration calculator transforms complex conversion factor calculations into an intuitive, professional-grade tool. With interactive examples, real-time validation, and comprehensive troubleshooting guidance, it's standardizing BCR-ABL1 monitoring across laboratories worldwide.

PUBLISHED: 10:00 AM, MAY 26, 2025 | BY LAB STATS TEAM
UNDERSTANDING IS

What is BCR-ABL1 IS Calibration?

What is IS Calibration?

The International Scale (IS) for BCR-ABL1 is like a universal language for laboratories worldwide. Think of it as converting different currencies to a single standard - just as you might convert pounds, euros, and dollars to a single currency for comparison.

Why is it Important?
  • Enables consistent patient monitoring across different laboratories
  • Critical for treatment decisions, especially when considering therapy cessation
  • Required for participation in clinical trials and research studies
Cross NCP et al. Development and evaluation of a secondary reference panel for BCR-ABL1 quantification on the International Scale. Leukemia (2016) 30, 1844-1852.
When Results Exceed Expected Values

If your calibration results deviate significantly from expected values:

  1. Check PCR Efficiency:
    • Optimal efficiency should be between 0.9 and 1.1
    • Efficiency ratios between BCR-ABL1 and control genes should be ~1
    • Suboptimal efficiency often indicates PCR optimization issues
  2. Verify Sample Input:
    • Use recommended RNA/cDNA input amounts
    • Check for PCR inhibition
    • Consider testing a dilution series
  3. Actions to Take:
    • Optimize PCR conditions (primers, temperatures, reagents)
    • Validate with additional control samples
    • Consider repeat testing with adjusted conditions
    • Document all troubleshooting steps
INSTRUCTIONS

How to Use This Calculator

  1. Select your control gene (ABL1, BCR, or GUSB)
  2. Enter your measured values for each MR level (MR1-MR4.5)
  3. Click "Calculate" to generate your conversion factor
  4. Review results and validation metrics
  5. Check the visualization for linearity
PRACTICE EXAMPLES

Interactive Examples

Optimal Calibration

Example of well-optimized assay results with excellent linearity and conversion factor accuracy.

Click to load example data
Suboptimal PCR

Example showing efficiency issues that require optimization before clinical use.

Click to load example data
Borderline Results

Example requiring careful evaluation and potential method optimization.

Click to load example data
Reference Values & Conversion Factors
ABL1 (CF: 0.93)
  • MR1 (A): 12.7575%
  • MR2 (B): 1.1942%
  • MR3 (C): 0.1019%
  • MR4 (D): 0.0108%
  • MR4.5 (E): 0.0038%
BCR (CF: 1.85)
  • MR1 (A): 21.3678%
  • MR2 (B): 1.3505%
  • MR3 (C): 0.1404%
  • MR4 (D): 0.0150%
  • MR4.5 (E): 0.0050%
GUSB (CF: 1.28)
  • MR1 (A): 11.7504%
  • MR2 (B): 0.7960%
  • MR3 (C): 0.0804%
  • MR4 (D): 0.0087%
  • MR4.5 (E): 0.0029%
CALCULATOR

Multi-point BCR-ABL1 IS Calibration Calculator